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anti pp1β  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti pp1β
    Anti Pp1β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pp1%CE%B2/PP1%CE%B2+Antibody/pmc12829218-50-50-52
    Average 92 stars, based on 36 article reviews
    anti pp1β - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: IIIG9 and PP1α form a protein complex in the adherens junctions of polarized ependymal and MDCK cells
    Article Snippet: .. In total, 10 μg, 15 μg, 20 μg, 50 μg, 80–100 μg of protein was loaded in each lane, separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (gradient 5–15%), transferred to PVDF membranes, and probed with anti-IIIG9 antibodies (1:10,000, in-house rabbit polyclonal antibody), anti-PP1α (1:2500, BD Transduction Laboratories, New York, USA), anti-PP1β (1:2000, Santa Cruz Biotechnology, CA, USA), anti-PP1γ (1:200, Santa Cruz Biotechnology, CA, USA), and anti-β-actin (1:10,000, Santa Cruz, CA, USA). .. Following incubation with an HRP-conjugated goat anti-rabbit IgG (1:5000; Jackson ImmunoResearch, Pennsylvania, USA) and HRP-conjugated goat anti-mouse IgG (1:5000; Jackson ImmunoResearch, Pennsylvania, USA).

    Western Blot:

    Article Title: pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread
    Article Snippet: The mass spectrometry proteomic data has been deposited with the ProteomeXchange Consortium ( http://www.proteomexchange.org/ ) via the PRIDE partner repository [ ] under the data set identifier PXD027257. .. The following antibodies with listed dilutions were used for immunoblotting: anti-CERT 1:10,000 (Abcam, ab72536), anti-pUL21 1:50 (this study, see below), anti-PP1α 1:1000 (Santa Cruz, sc-271762), anti-PP1β 1:1000 (Santa Cruz, sc-373782), anti-PP1γ 1:1000 (Santa Cruz, sc-6108), anti-pUL34 1:500 [ ], anti-pUL31 1:200 [ ], anti-pUS3 1:1000 [ ], anti-VP5 [DM165] 1:50 [ ], mouse anti-GAPDH 1:10,000 (GeneTex, GTX28245), rabbit anti-GAPDH 1:10,000 (GeneTex, GTX100118), anti-pUL16 1:2000 [ ], anti-phospho-PKA substrates 1:1000 (Cell Signalling, 9624), anti-phospho-Akt substrates 1:1000 (Cell Signalling, 9611). .. After incubation with the 1:10,000 dilutions of fluorescently labelled secondary antibodies: LI-COR IRDye 680T conjugated goat anti-rat (926–68029), donkey anti-rabbit (926–68023) or goat anti-mouse (926–68020), or LI-COR IRDye 800CW conjugated donkey anti-rabbit (926–32213), donkey anti-goat (926–32214), donkey anti-chicken (926–32218) or goat anti-mouse (926–32210), the signals were detected using Odyssey CLx Imaging System (LI-COR) and analysed using Image Studio Lite software (LI-COR).

    Article Title: Dok3–protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity
    Article Snippet: Cell lysates were IP overnight and pulled down using Protein A/G Plus Agarose beads (Santa Cruz Biotechnology Inc.). .. Precipitates were analyzed by Western blotting according to standard protocol using the indicated antibodies: anti-Card9 (catalog 12283, Cell Signaling Technology; clone A-8, Santa Cruz Biotechnology Inc.), anti-Dok3 (clone E-17, M-214, H-5; Santa Cruz Biotechnology Inc.), anti–p-Erk1/2 T202/Y204 (catalog 4370; Cell Signaling Technology), anti-Erk2 (clone C-14; Santa Cruz Biotechnology Inc.), anti–p-IKKα/β S176/180 (catalog 2697; Cell Signaling Technology), anti-IKKα/β (clone H-470; Santa Cruz Biotechnology Inc.), anti–p-JNK T183/Y185 (catalog 9255; Cell Signaling Technology), anti-JNK (catalog 9252; Cell Signaling Technology), anti–p-p38 T180/Y182 (clone D-8; Santa Cruz Biotechnology Inc.), anti-p38 (clone N-20; Santa Cruz Biotechnology Inc.), anti–p-Syk Y352 (catalog 2717; Cell Signaling Technology), anti-Syk (catalog 80460; Cell Signaling Technology), anti–p-Thr (catalog 9381; Cell Signaling Technology), anti–p-Tyr (clone 4G10; Millipore), anti–β-actin (clone ACTBD11B7; Santa Cruz Biotechnology Inc.), anti-PP1 (clone E-9; Santa Cruz Biotechnology Inc.), anti-PP2AC (catalog 2259; Cell Signaling Technology), anti-PP1α (clone G-4; Santa Cruz Biotechnology Inc.), anti-PP1β (clone A-6; Santa Cruz Biotechnology Inc.), anti-PP1γ (clone E-4; Santa Cruz Biotechnology Inc.), anti–p-PKCδ Y311 (clone A-8;. .. Santa Cruz Biotechnology Inc.), and anti-PKCδ (clone G-9; Santa Cruz Biotechnology Inc.).

    Article Title: pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread
    Article Snippet: The mass spectrometry proteomic data has been deposited with the ProteomeXchange Consortium ( http://www.proteomexchange.org/ ) via the PRIDE partner repository under the data set identifier PXDxxxxxx ( http://www.ebi.ac.uk/pride/archive/projects/PXDxxxxxx ). .. The following antibodies with listed dilutions were used for immunoblotting: anti-CERT 1:10,000 (Abcam, ab72536), anti-pUL21 1:50 (this study, see below), anti-PP1α 1:1000 (Santa Cruz, sc-271762), anti-PP1β 1:1000 (Santa Cruz, sc-373782), anti-PP1γ 1:1000 (Santa Cruz, sc-6108), anti-pUL34 1:500 , anti-pUL31 1:200 , anti-pUS3 1:1000 , anti-VP5 [DM165] 1:50 , mouse anti-GAPDH 1:10,000 (GeneTex, GTX28245), rabbit anti-GAPDH 1:10,000 (GeneTex, GTX100118), anti-pUL16 1:2000 , anti-phospho-PKA substrates 1:1000 (Cell Signalling, 9624), anti-phospho-Akt substrates 1:1000 (Cell Signalling, 9611). .. After incubation with the 1:10,000 dilutions of fluorescently labelled secondary antibodies: LI-COR IRDye 680T conjugated goat anti-rat (926-68029), donkey anti-rabbit (926-68023) or goat anti-mouse (926-68020), or LI-COR IRDye 800CW conjugated donkey anti-rabbit (926-32213), donkey anti-goat (926-32214), donkey anti-chicken (926-32218) or goat anti-mouse (926-32210), the signals were detected using Odyssey CLx Imaging System (LI-COR) and analysed using Image Studio Lite software (Li-COR).

    Article Title: Dok3–protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity
    Article Snippet: Cell lysates were IP overnight and pulled down using Protein A/G Plus Agarose beads (Santa Cruz Biotechnology Inc.). .. Precipitates were analyzed by Western blotting according to standard protocol using the indicated antibodies: antiCard9 (catalog 12283, Cell Signaling Technology; clone A-8, Santa Cruz Biotechnology Inc.), anti-Dok3 (clone E-17, M-214, H-5; Santa Cruz Biotechnology Inc.), anti–p-Erk1/2T202/Y204 (catalog 4370; Cell Signaling Technology), anti-Erk2 (clone C-14; Santa Cruz Biotechnology Inc.), anti–p-IKKα/βS176/180 (catalog 2697; Cell Signaling Technology), anti-IKKα/β (clone H-470; Santa Cruz Biotechnology Inc.), anti–p-JNKT183/Y185 (catalog 9255; Cell Signaling Technology), anti-JNK (catalog 9252; Cell Signaling Technology), anti–p-p38T180/Y182 (clone D-8; Santa Cruz Biotechnology Inc.), anti-p38 (clone N-20; Santa Cruz Biotechnology Inc.), anti–p-SykY352 (catalog 2717; Cell Signaling Technology), anti-Syk (catalog 80460; Cell Signaling Technology), anti– p-Thr (catalog 9381; Cell Signaling Technology), anti–p-Tyr (clone 4G10; Millipore), anti–β-actin (clone ACTBD11B7; Santa Cruz Biotechnology Inc.), anti-PP1 (clone E-9; Santa Cruz Biotechnology Inc.), anti-PP2AC (catalog 2259; Cell Signaling Technology), anti-PP1α (clone G-4; Santa Cruz Biotechnology Inc.), anti-PP1β (clone A-6; Santa Cruz Biotechnology Inc.), anti-PP1γ (clone E-4; Santa Cruz Biotechnology Inc.), anti– p-PKCδY311 (clone A-8;. .. Santa Cruz Biotechnology Inc.), and anti-PKCδ (clone G-9; Santa Cruz Biotechnology Inc.).

    Incubation:

    Article Title: CAVPENET Peptide Inhibits Prostate Cancer Cells Proliferation and Migration through PP1γ-Dependent Inhibition of AKT Signaling
    Article Snippet: .. The primary antibodies were diluted in either 5%non-fat milk or 5% BSA and incubated for 1 h or ON at 4 °C: anti-p(Ser473)-AKT (rabbit, 1:2000, 4060S, Cell Signaling), anti-AKT (mouse, 1:500, sc-5298, Santa Cruz Technologies, Santa Cruz, CA, USA), anti-p(Ser9)-GSK3β (mouse, 1:1000, sc-373800, Santa Cruz Biotechnologies, Santa Cruz, CA, USA), anti-GSK3β (rabbit, 1:1000, 9315S, Cell Signaling), anti-p(Thr320)-PP1α (rabbit, 1:1000, ab62334, Abcam, Cambridge, UK), anti-PP1α (rabbit, 1:2500, homemade), anti-PP1β (mouse, 1:1000, sc-365678, Santa Cruz Biotechnologies), anti-PP1γ (rabbit, 1:1000, homemade), anti-p(Thr37/46)-4E-BP1 (rabbit, 1:1000, 236B4, Cell Signaling), anti-4E-BP1 (rabbit, 1:1000), anti-HK2 (mouse, 1:500, sc-374091, Santa Cruz Biotechnologies), anti-FASN (mouse, 1:500, sc-55580, Santa Cruz Biotechnologies); anti-CD36 (rabbit, 1:2000, NB400-144, Novus Biologicals, Centennial, CO, USA) and anti-CPT1A (mouse, 1:500, sc-393070, Santa Cruz Biotechnologies). .. Then, the membranes were washed three times with TBS-T, 10 min and incubated with the respective secondary antibodies IRDye ® 68RD anti-rabbit (926-68071), 1:10,000 and IRDye ® 800CW anti-mouse (926-32210), 1:10,000, LI-COR Biosciences (Licon, NE, USA) for 1 h at RT.



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